5' RACE by tailing a general template-switching oligonucleotide.
نویسندگان
چکیده
منابع مشابه
Characterization of the extreme 5' ends of RNA molecules by RNA ligation-PCR.
1Permanent address: P..O. Box 677, Nedlands, Western Australia, 6009. Despite major recent advances in PCRbased methodology, characterization of the extreme terminal sequences of RNA molecules may be difficult and time consuming, particularly when the 3' end is not polyadenylated.~l~ The usual approach to defining the 5' end involves cDNA synthesis using gene-specific downstream priming of the ...
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Rapid amplification of cDNA 5′ ends (5′-RACE) is a convenient way to retrieve information about the 5′ termini of mRNAs because entire open reading frames are difficult to predict from genomic information and expressed sequence tag libraries generally lack full 5′ termini. To achieve this, numerous methods have been developed, each of which has been successful in specific situations (3–5,11–14,...
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ورودعنوان ژورنال:
- BioTechniques
دوره 29 6 شماره
صفحات -
تاریخ انتشار 2000